Vol.59 No.1 January 2011
Effect of antibiotic combinations by "Break-point Checkerboard Plate" against multidrug-resistant Pseudomonas aeruginosa
1)Department of Clinical Laboratory, Shiga Medical Center for Adults, 5-4-30 Moriyama, Moriyama, Shiga, Japan
2)Central Laboratory Technical Section 3., FALCO biosystems Ltd.
3)Department of Clinical Laboratory, Social Insurance Shiga Hospital
4)Department of Laboratory Medicine, Otsu Red Cross Hospital
5)Department of Clinical Laboratory, Kobe University Hospital
Abstract
We studied antibiotic combination effects by "Break-point Checkerboard Plate" against multiple-drug-resistant Pseudomonas aeruginosa(MDRP). Fifty-four MDRP strains collected from October 2000 to April 2007 from clinical laboratories in Kinki, Japan were tested using Break-point Checkerboard Plates to evaluate the effect with combined antibiotics. Amikacin plus aztreonam and colistin plus rifampicin showed a synergistic effect of 31/48 and 32/32. We concluded that these plates are useful in screening antibiotic combination effects in the clinical setting.
Key word
multidrug-resistant Pseudomonas aeruginosa, synergy, breakpoint, checkerboard titration method
Received
March 12, 2010
Accepted
September 8, 2010
Jpn. J. Chemother. 59 (1): 29-33, 2011
